Well, it worked, but they’ve pretty much blocked it from their memory since then.
It’s disgusting to imagine that Peter accidentally caused his true love’s death with his, er, DNA, and it comes across as a cheap way to kill Mary Jane with enough shock value to catch the audience’s attention. It is eventually revealed that his beloved Mary Jane died from cancer brought on by Peter’s radioactive sperm. In what is supposed to be Spider-Man’s version of The Dark Knight Returns, Spider-Man: Reign portrayed the hero as a retired old man living alone in a dystopian New York.
Spider-Man once killed Mary Jane! Marvel Comics